灵长类疾病模型研究组（杨辉组）生物材料共享信息--中国科学院脑科学与智能技术卓越创新中心

灵长类疾病模型研究组（杨辉组）生物材料共享信息

发布时间：2019-12-17

　　Plasmids Available From Addgene

　　You can obtain reagents via the nonprofit plasmid repository Addgene.

　　If you cannot obtain reagents through Addgene, please feel free to reach out to us directly.

ID	Plasmid	Gene/Insert	Vector Type	Publication
97307	AAV_Efs_hSpCas9_NLS_FLAG-SV40	humanized S. pyogenes Cas9	Mammalian Expression, Mouse Targeting, AAV	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97308	AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA	Actb HMEJ donor	Mammalian Expression, Mouse Targeting, AAV	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97309	AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA	Actb HR donor	Mammalian Expression, Mouse Targeting, AAV	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97310	AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA	Actb NHEJ donor	Mammalian Expression, Mouse Targeting, AAV	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97311	AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA	Actb MMEJ donor	Mammalian Expression, Mouse Targeting, AAV	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97312	Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA	Actb HMEJ donor	Mammalian Expression, Mouse Targeting, Lentiviral	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97313	Lenti_Efs_hSpCas9_NLS_FLAG-WPRE	humanized S. pyogenes Cas9	Mammalian Expression, Mouse Targeting, Lentiviral	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97316	AAV_Actb NHEJ donor	Actb NHEJ donor	Mammalian Expression, Mouse Targeting	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97317	AAV_Actb HR donor	Actb HR donor	Mammalian Expression, Mouse Targeting	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97318	AAV_Actb HMEJ donor	Actb HMEJ donor	Mammalian Expression, Mouse Targeting	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97319	AAV_Cdx2 HMEJ donor	Cdx2 HMEJ donor	Mammalian Expression, Mouse Targeting	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97320	AAV_Dbh HMEJ donor	Dbh HMEJ donor	Mammalian Expression, Mouse Targeting	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97321	AAV_Nanog HMEJ donor	Nanog HMEJ donor	Mammalian Expression, Mouse Targeting	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
97322	AAV_Sox2 HMEJ donor	Sox2 HMEJ donor	Mammalian Expression, Mouse Targeting	Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
107307	CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA	CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA	Mammalian Expression	In vivosimultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6.
107310	dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4	dSV40-dCas9-10xGCN4	Mammalian Expression, Lentiviral	In vivosimultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6.
107311	EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA	EF1_-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA	Mammalian Expression, Lentiviral	In vivosimultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6.
107312	AAV-hSyn-mCherry-P2A-Cre-WPRE	hSyn-mCherry-P2A-Cre-WPRE	Mammalian Expression, AAV	In vivosimultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6.
107313	AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA	aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA	Mammalian Expression, AAV	In vivosimultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6.
154000	AAV-GFAP-NLS-CasRx-NLS-FLAG	CasRx	CRISPR	Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. Cell. 2020 Apr 30;181(3):590-603.e16. doi: 10.1016/j.cell.2020.03.024. Epub 2020 Apr 8.
154001	AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR	CasRx	Mouse Targeting, CRISPR	Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. Cell. 2020 Apr 30;181(3):590-603.e16. doi: 10.1016/j.cell.2020.03.024. Epub 2020 Apr 8.
154002	PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40	SgRNA expression system	CRISPR	Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver. Protein Cell. 2020 Mar 18. pii: 10.1007/s13238-020-00700-2. doi: 10.1007/s13238-020-00700-2.
154003	AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR	CasRx	CRISPR	Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver. Protein Cell. 2020 Mar 18. pii: 10.1007/s13238-020-00700-2. doi: 10.1007/s13238-020-00700-2.
154004	PB-CAG-CasRX-P2A-EGFP-BGH PolyA	CasRx	CRISPR	Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver. Protein Cell. 2020 Mar 18. pii: 10.1007/s13238-020-00700-2. doi: 10.1007/s13238-020-00700-2.
154005	CMV-YE1-BE3-FNLS-CMV-mCherry	YE1-BE3-FNLS	CRISPR	A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects. Nat Methods. 2020 May 18. pii: 10.1038/s41592-020-0832-x. doi: 10.1038/s41592-020-0832-x.

　　Transgenic Mice

Stock No.	Name	Other Called	Publication
Stock No. at Jackson Lab 031645	B6;D2-Gm33925Tn(pb-CAG-cas9*,-EGFP)1Yangh/J	Cre-dependent SPH (SunTag-p65-HSF1)	In vivosimultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6.
Stock No. at Shanghai Model Organisms NM-TG-00025	C57BL/6-Tg(CAG-LSL-dCas9-SPH)Smoc	CAG-LSL-dCas9-SPH	In vivosimultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6.

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