目录Menu 

　　行为学装置Behavioral setup 

　　场景认知行为Linear track setup 

　　虚拟现实VR setup 

　　恐惧学习记忆Fear conditioning setup 

　　神经活动记录和操控Neural recording and manipulation 

　　多通道电极记录Multi-channel single-unit recording 

　　内窥式内置显微镜UCLA miniscope 

　　光纤记录系统Photometric recording 

　　膜片钳Patch-clamp recording 

　　在体单细胞记录Juxtacellular recording 

　　光遗传操控Optogenetics 

　　通用设备Common setups 

　　荧光显微镜Fluorescence microscope 

　　立体定位注射Stereotaxic injection 

　　分克克隆Molecular Cloning 

　　神经环路示踪Neural circuit tracing 

　　跨突触示踪Rabies based trans-synaptic tracing 

　　多条件控制的基因表达Intersectional control 

行为学装置Behavioral setup

场景认知行为Linear track setup

　　·        Fully automatic control of behavioral training 

　　·        Interleaved trials for context switches 

　　·        Context-dependent tasks in behaving animals 

虚拟现实VR setup

　　·        Rodent running on a spherical treadmill 

　　·        270° TFT monitor (or 150° curved back-projecting screen) for presenting virtual reality 

　　·        Custom behavioral training with licking reward and air-puff punishment 

　　·        Compatible with 2-photo microscopy imaging  

　　·        Compatible with miniscope Ca²⁺ imaging 

恐惧学习记忆Fear conditioning setup

　　·        Custom software to acquire behavioral video and trigger optogenetic devices 

　　·        Compatible with photometry and miniscope recording 

神经活动记录和操控Neural recording and manipulation

多通道电极记录Multi-channel single-unit recording

　　·        64 channel acquisition systems from BlackRock and Open-Ephy 

　　·        Tetrode recording in free-moving animals 

　　·        Spike sorting using OFSS 

内窥式内置显微镜UCLA miniscope

　　·        > 1mm diameter field of view and ~ 1mm working distance 

　　·        +/- 200um electronic focal adjustment 

　　·        2.6 grams and 22 mm tall 

　　·        Absolute head orientation sensor 

　　(Courtesy of Miniscope-v4 wiki. We are using v2 in the lab for now) 

光纤记录系统Photometric recording

　　·        Multi-Fiber Photometry System (4 channels) 

　　·        Integrated with behavioral video acquisition and analysis 

　　·        Synchronized with external equipment (such as optogenetics) via TTL 

膜片钳Patch-clamp recording

　　·        Axon 700A/700B, Digidata 1440, pClamp10. 

　　·        Sutter Motorized Micromanipulator, Nikon microscope, Thorlab CCD. 

在体单细胞记录Juxtacellular recording

　　·        npi amplifier ELC-03XS, CED Micro 1401 

光遗传操控Optogenetics

　　·        462 nm diode blue laser, 589 nm DPSS yellow laser 

　　·        Adjustable power, TTL trigger mode 

通用设备Common setups

荧光显微镜Fluorescence microscope

　　·        Blue, Green, Red and Far-red channels  

　　·        Equipped with motorized platform and NeuroLucida 8 

立体定位注射Stereotaxic injection

　　·        Digital display of stereotaxic coordinates 

　　·        Precise control of isoflurane 

　　·        Fast anesthesia and fast recovery 

分子克隆Molecular Cloning

　　·        Standard equipment for cloning  

神经环路示踪Neural circuit tracing

跨突触示踪Rabies based trans-synaptic tracing

　　·        Cell-type specific retrograde trans-synaptic tracing 

　　·        Example below showing projection-cell-specific tracing of vHC-CEA-PAG pathway 

　　·        Quantitative analysis of circuit tracing in the whole-brain scale 

多条件控制的基因表达Intersectional control

　　·        IBIST (intein based intersectional synthesis of transactivator) 

　　·        Intersectional expression for ChR2 and GCamP6 in tTA-dependent manner